1QCS
N-TERMINAL DOMAIN OF N-ETHYLMALEIMIDE SENSITIVE FACTOR (NSF)
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.2 |
Synchrotron site | ALS |
Beamline | 5.0.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 1998-02-13 |
Detector | ADSC |
Wavelength(s) | 0.9799, 0.9801, 0.9998, 0.9537 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 32.042, 175.362, 79.663 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.000 - 1.900 |
R-factor | 0.21 * |
Rwork | 0.210 |
R-free | 0.24200 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.420 * |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.970 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.057 | 0.270 * |
Number of reflections | 33216 * | |
<I/σ(I)> | 20.4 | |
Completeness [%] | 98.0 | 92.7 |
Redundancy | 3.2 | 2.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, sitting drop * | 8.7 | 20 * | Vapor diffusion, hanging drop, 100 mM Tris pH 8.7, 2.0 M ammonium sulfate, 10 mM dithiothreitol (DTT), VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 10 (mg/ml) | |
2 | 1 | drop | Tris-HCl | 100 (mM) | |
3 | 1 | drop | ammonium sulfate | 2.0 (M) | |
4 | 1 | drop | dithiothreitol | 10 (mM) | |
5 | 1 | reservoir | Tris-HCl | 100 (mM) | |
6 | 1 | reservoir | ammonium sulfate | 2.0 (M) | |
7 | 1 | reservoir | dithiothreitol | 10 (mM) |