1QCR
CRYSTAL STRUCTURE OF BOVINE MITOCHONDRIAL CYTOCHROME BC1 COMPLEX, ALPHA CARBON ATOMS ONLY
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X12B |
| Synchrotron site | NSLS |
| Beamline | X12B |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1995-07 |
| Detector | MARRESEARCH |
| Spacegroup name | I 41 2 2 |
| Unit cell lengths | 153.500, 153.500, 597.700 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 2.700 |
| R-factor | 0.3 |
| Rwork | 0.300 |
| R-free | 0.37500 |
| Structure solution method | MIR |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR (3.851) |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 2.970 |
| High resolution limit [Å] | 2.700 | 2.900 |
| Rmerge | 0.145 | 0.584 |
| Total number of observations | 2166501 * | |
| Number of reflections | 72196 | |
| <I/σ(I)> | 10.4 | 1 |
| Completeness [%] | 90.6 | 62.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | unknown * | 7.2 | pH 7.2 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | complex | 20 (mg/ml) | |
| 10 | 1 | 2 | detergent | 0.1 (%) | |
| 2 | 1 | 1 | MOPS | 50 (mM) | |
| 3 | 1 | 1 | ammonium acetate | 20 (mM) | |
| 4 | 1 | 1 | glycerol | 20 (%) | |
| 5 | 1 | 1 | DMG | 0.1 (%) | can be replaced by 0.1% SPC |
| 6 | 1 | 2 | PEG4000 | 12 (%) | |
| 7 | 1 | 2 | MOPS | 50 (mM) | |
| 8 | 1 | 2 | 0.5 (M) | ||
| 9 | 1 | 2 | glycerol | 20 (%) |
