1QCD
CRYSTAL STRUCTURES OF ADENINE PHOSPHORIBOSYLTRANSFERASE FROM LEISHMANIA DONOVANI
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 298 |
| Detector technology | AREA DETECTOR |
| Collection date | 1995-09-01 |
| Detector | OTHER |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 64.040, 64.040, 240.530 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 18.570 - 2.480 |
| Rwork | 0.174 |
| R-free | 0.25000 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 24.900 * |
| Data reduction software | SDMS |
| Data scaling software | SDMS |
| Phasing software | PHASES |
| Refinement software | X-PLOR (3.843) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 18.600 * | |
| High resolution limit [Å] | 2.480 * | |
| Rmerge | 0.060 * | 0.149 * |
| Total number of observations | 33527 * | |
| Number of reflections | 9680 * | |
| Completeness [%] | 87.2 * | 50.1 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 * | 277 | AMMONIUM SULFATE, SODIUM CITRATE, MAGNESIUM CHLORIDE, pH 4.9, VAPOR DIFFUSION, HANGING DROP, temperature 277.0K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | drop | MES | 10 (mM) | |
| 3 | 1 | drop | dithiothreitol | 1 (mM) | |
| 4 | 1 | drop | 5 (mM) | ||
| 5 | 1 | reservoir | ammonium sulfate | 1.2-1.6 (M) | |
| 6 | 1 | reservoir | sodium citrate | 0.1 (M) | |
| 7 | 1 | reservoir | 10 (mM) |
