1QC6
EVH1 domain from ENA/VASP-like protein in complex with ACTA peptide
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X9B |
| Synchrotron site | NSLS |
| Beamline | X9B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1999-02-15 |
| Detector | ADSC |
| Wavelength(s) | 0.980544, 0.978455, 0.941310 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 44.883, 68.257, 82.219 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 2.600 |
| R-factor | 0.225 |
| Rwork | 0.215 |
| R-free | 0.29800 |
| Structure solution method | MAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 28.910 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.690 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.090 | 0.298 |
| Number of reflections | 7926 | |
| <I/σ(I)> | 18.3 | 4.8 |
| Completeness [%] | 96.6 | 88.1 |
| Redundancy | 2.4 | 1.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | ammonium sulfate, DTT, sodium azide, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10-14 (mg/ml) | |
| 2 | 1 | reservoir | ammonium sulfate | 1.7 (M) | |
| 3 | 1 | reservoir | dithiothreitol | 5 (mM) | |
| 4 | 1 | reservoir | 0.02 (%) |
