1QC5
I Domain from Integrin Alpha1-Beta1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RUH3R |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 1998-11-09 |
Detector | RIGAKU RAXIS IV |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 37.440, 96.350, 53.270 |
Unit cell angles | 90.00, 104.28, 90.00 |
Refinement procedure
Resolution | 100.000 - 2.000 |
R-factor | 0.206 * |
Rwork | 0.206 |
R-free | 0.24300 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | THE A DOMAIN OF HUMAN COMPLEMENT FACTOR B |
RMSD bond length | 0.007 |
RMSD bond angle | 1.200 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS (0.4) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 100.000 | 2.070 |
High resolution limit [Å] | 2.000 | 1.990 |
Rmerge | 0.061 | 0.209 |
Total number of observations | 140776 * | |
Number of reflections | 24698 | |
<I/σ(I)> | 24.1 | 8.1 |
Completeness [%] | 99.2 | 95.3 |
Redundancy | 5.7 | 4.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 * | 298 | MMEPEG2000, HEPES, SODIUM CHLORIDE, MAGNESIUM CHLORIDE, BETA-MERCAPTOETHANOL, pH 7.50, VAPOR DIFFUSION, HANGING DROP, temperature 298.00K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 20 (mg/ml) | |
10 | 1 | reservoir | beta-mercaptoethanol | 5 (mM) | |
2 | 1 | drop | HEPES | 10 (mM) | |
3 | 1 | drop | 200 (mM) | ||
4 | 1 | drop | 5 (mM) | ||
5 | 1 | drop | beta-mercaptoethanol | 5 (mM) | |
6 | 1 | reservoir | PEG2000 | 31 (%) | |
7 | 1 | reservoir | HEPES | 50 (mM) | |
8 | 1 | reservoir | 200 (mM) | ||
9 | 1 | reservoir | 5 (mM) |