1QBZ
THE CRYSTAL STRUCTURE OF THE SIV GP41 ECTODOMAIN AT 1.47 A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X9B |
| Synchrotron site | NSLS |
| Beamline | X9B |
| Temperature [K] | 93 |
| Detector technology | IMAGE PLATE |
| Collection date | 1997-11-22 |
| Detector | MARRESEARCH |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 91.124, 47.206, 77.465 |
| Unit cell angles | 90.00, 100.83, 90.00 |
Refinement procedure
| Resolution | 15.000 - 1.470 |
| R-factor | 0.152 |
| R-free | 0.21800 |
| Structure solution method | MIR AND MR |
| Starting model (for MR) | POLY ALANINE MODEL (RESIDUES 46-74) OF MMLV TRANSMEMBRANE TRIMERIC CORE (1MOF) |
| RMSD bond length | 0.011 |
| RMSD bond angle | 0.030 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MLPHARE (PHASES DM AMORE) |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.000 | 1.500 |
| High resolution limit [Å] | 1.470 | 1.470 |
| Rmerge | 0.064 | 0.190 |
| Total number of observations | 412245 * | |
| Number of reflections | 49553 | |
| <I/σ(I)> | 14.8 | 3.6 |
| Completeness [%] | 89.9 | 78.7 * |
| Redundancy | 8.3 | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, sitting drop * | 3 * | 14-25% MPD (2-METHYL-2,4- PENTANEDIOL) 140-220 MM NACL, 20-40 MM SODIUM ACETATE, pH 4.25 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10-15 (mg/ml) | |
| 2 | 1 | drop | sodium formate | 50 (mM) | |
| 3 | 1 | reservoir | MPD | 14-25 (%(v/v)) | |
| 4 | 1 | reservoir | 140-220 (mM) | ||
| 5 | 1 | reservoir | sodium acetate | 20-40 (mM) |
