1QBP
CRYSTAL STRUCTURE OF A BROMINATED RNA HELIX WITH FOUR MISMATCHED BASE PAIRS
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL1-5 |
| Synchrotron site | SSRL |
| Beamline | BL1-5 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1996-02-25 |
| Detector | FUJI |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 104.450, 24.270, 104.450 |
| Unit cell angles | 90.00, 118.76, 90.00 |
Refinement procedure
| Resolution | 9999.000 * - 2.100 |
| R-factor | 0.217 |
| Rwork | 0.217 |
| R-free | 0.25300 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.210 |
| Data reduction software | DENZO |
| Data scaling software | CCP4 ((TRUNCATE)) |
| Phasing software | MLPHARE |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 30.000 |
| High resolution limit [Å] | 2.100 |
| Rmerge | 0.060 |
| Number of reflections | 11919 |
| <I/σ(I)> | 11.5 |
| Completeness [%] | 99.0 |
| Redundancy | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 292 | 25 MM SODIUM CACODYLATE, 45 MM MGCL2, 60 MM SPERMINE, 6.7 % MPD, RESERVOIR 40 % MPD, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 292K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | SODIUM CACODYLATE | ||
| 2 | 1 | 1 | MGCL2 | ||
| 3 | 1 | 1 | SPERMINE | ||
| 4 | 1 | 1 | MPD | ||
| 5 | 1 | 2 | MPD |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | RNA | 0.5 (mM) | |
| 2 | 1 | drop | sodium cacodylate | 25 (mM) | |
| 3 | 1 | drop | 40-50 (mM) | ||
| 4 | 1 | drop | spermine | 60 (mM) | |
| 5 | 1 | drop | 6.7 (%) | ||
| 6 | 1 | reservoir | MPD | 40 (%) |
