1QB8
CRYSTAL STRUCTURES OF ADENINE PHOSPHORIBOSYLTRANSFERASE FROM LEISHMANIA DONOVANI
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL7-1 |
Synchrotron site | SSRL |
Beamline | BL7-1 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 1997-03-02 |
Detector | MARRESEARCH |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 64.040, 64.040, 235.950 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 19.000 - 2.000 |
Rwork | 0.196 |
R-free | 0.26300 |
RMSD bond length | 0.007 |
RMSD bond angle | 24.500 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASES |
Refinement software | X-PLOR (3.843) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 19.000 * | |
High resolution limit [Å] | 2.000 * | |
Rmerge | 0.053 * | 0.175 * |
Total number of observations | 328256 * | |
Number of reflections | 17882 * | |
Completeness [%] | 87.9 * | 60.3 * |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 * | 4 * | PEG 5000 MME, AMMONIUM ACETATE, SODIUM CITRATE, MAGNESIUM CHLORIDE, ADENOSINE- 5-MONOPHOSPHATE (AMP), pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 277.0K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 10 (mg/ml) | |
2 | 1 | drop | MES | 10 (mM) | |
3 | 1 | drop | dithiothreitol | 1 (mM) | |
4 | 1 | drop | 5 (mM) | ||
5 | 1 | reservoir | AMP | 10 (mM) | |
6 | 1 | reservoir | mPEG5000 | 7-11 (%) | |
7 | 1 | reservoir | ammonium acetate | 0.2 (M) | |
8 | 1 | reservoir | sodium citrate | 0.1 (M) | |
9 | 1 | reservoir | 10 (mM) |