1QB3
CRYSTAL STRUCTURE OF THE CELL CYCLE REGULATORY PROTEIN CKS1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL7-1 |
| Synchrotron site | SSRL |
| Beamline | BL7-1 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1995-10-20 |
| Detector | MARRESEARCH |
| Spacegroup name | P 62 2 2 |
| Unit cell lengths | 105.810, 105.810, 165.880 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 12.000 - 3.000 |
| R-factor | 0.216 * |
| Rwork | 0.216 |
| R-free | 0.29100 |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.900 |
| Data reduction software | DENZO |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 12.000 | 3.100 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.094 | 0.405 |
| Total number of observations | 243609 * | |
| Number of reflections | 10366 * | |
| <I/σ(I)> | 7.4 | |
| Completeness [%] | 91.0 | 93.7 |
| Redundancy | 12.6 | 11.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 7 | 20 * | 20% PEG 4K, 5% NASCN AND 0.1 M IMIDAZOLE/MALATE, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 12 (mg/ml) | |
| 2 | 1 | drop | PEG4000 | 20 (%) | |
| 3 | 1 | drop | 5 (%sat) | ||
| 4 | 1 | drop | imidazole/malate | 0.1 (M) |
