1QAZ
CRYSTAL STRUCTURE OF ALGINATE LYASE A1-III FROM SPHINGOMONAS SPECIES A1 AT 1.78A RESOLUTION
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | MACSCIENCE |
| Temperature [K] | 293 |
| Detector technology | AREA DETECTOR |
| Collection date | 1998-10-23 |
| Detector | SIEMENS HI-STAR |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 48.900, 92.400, 81.600 |
| Unit cell angles | 90.00, 104.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 1.780 |
| Rwork | 0.180 |
| R-free | 0.23300 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 2.670 |
| Data reduction software | SADIE |
| Data scaling software | SADIE |
| Phasing software | PHASES |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 26.310 | 1.840 |
| High resolution limit [Å] | 1.790 | 1.780 |
| Rmerge | 0.050 * | |
| Total number of observations | 127587 * | |
| Number of reflections | 34661 * | |
| <I/σ(I)> | 7.9 | |
| Completeness [%] | 90.2 * | 49.9 |
| Redundancy | 2.12 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 20 * | drop consists of equal volume of protein and reservoir solutions * |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | (NH4)2SO4 | ||
| 2 | 1 | 1 | HEPES | ||
| 3 | 1 | 2 | (NH4)2SO4 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 17.2 (mg/ml) | |
| 2 | 1 | drop | 0.1 (M) | ||
| 3 | 1 | reservoir | ammonium sulfate | 47-49 (%sat) | |
| 4 | 1 | reservoir | HEPES | 0.1 (M) |
