1QAX
TERNARY COMPLEX OF PSEUDOMONAS MEVALONII HMG-COA REDUCTASE WITH HMG-COA AND NAD+
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 295 |
| Detector technology | IMAGE PLATE |
| Collection date | 1997-01-01 |
| Detector | RIGAKU RAXIS II |
| Spacegroup name | I 41 3 2 |
| Unit cell lengths | 228.700, 228.700, 228.700 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 2.800 |
| R-factor | 0.194 |
| Rwork | 0.215 |
| R-free | 0.26700 |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.850 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.071 | 0.300 |
| Number of reflections | 23346 | |
| <I/σ(I)> | 15 | |
| Completeness [%] | 92.2 | 64.6 |
| Redundancy | 4 | 1.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 8.2 * | VAPOR DIFFUSION, SITTING DROP, 295K, PH 6.7, 1.2M AMMONIUM SULFATE, 100MM ADA BUFFER |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | Tris-HCl | 10 (mM) | |
| 2 | 1 | drop | 400 (mM) | ||
| 3 | 1 | drop | glycerol | 10 (%) | |
| 4 | 1 | reservoir | ammonium sulfate | 1.2 (M) | |
| 5 | 1 | reservoir | sodium citrate | 75 (mM) | |
| 6 | 1 | reservoir | sodium ADA | 100 (mM) |
