1QAW
Regulatory Features of the TRP Operon and the Crystal Structure of the TRP RNA-Binding Attenuation Protein from Bacillus Stearothermophilus.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE BW7B |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | BW7B |
Temperature [K] | 120 |
Detector technology | IMAGE PLATE |
Collection date | 1996-01-01 |
Detector | MARRESEARCH |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 65.930, 117.960, 147.630 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 15.000 - 2.500 |
Rwork | 0.215 |
R-free | 0.29900 |
RMSD bond length | 0.014 |
RMSD bond angle | 0.043 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 15.000 | 2.540 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.089 | 0.487 |
Number of reflections | 36543 | |
<I/σ(I)> | 14.2 | |
Completeness [%] | 90.5 | 90 |
Redundancy | 4 | 3.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.8 | 18 * | d * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 18 (mg/ml) | |
2 | 1 | reservoir | sodium phosphate | 0.1 (M) | precipitant |
3 | 1 | reservoir | mPEG2000 | precipitant | |
4 | 1 | reservoir | 4 (M) | ||
5 | 1 | drop | sodium phosphate | 0.1 (M) | |
6 | 1 | drop | mPEG2000 |