1QAU

UNEXPECTED MODES OF PDZ DOMAIN SCAFFOLDING REVEALED BY STRUCTURE OF NNOS-SYNTROPHIN COMPLEX

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL7-1
Synchrotron site	SSRL
Beamline	BL7-1
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1998-04-23
Detector	MARRESEARCH
Spacegroup name	P 21 21 21
Unit cell lengths	35.180, 38.990, 85.600
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 1.250
Rwork	0.239
R-free	0.25500 *
RMSD bond length	0.004
RMSD bond angle	1.390
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHARP
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.290
High resolution limit [Å]	1.250	1.250
Rmerge	0.038	0.290
Total number of observations	131703 *
Number of reflections	33078
<I/σ(I)>	32.1
Completeness [%]	98.9	99
Redundancy	4	3.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	8.5	298	Vapor diffusion, sitting drop, pH 8.5, 298K, 15% PEG 4000, 100 mM Tris , VAPOR DIFFUSION, SITTING DROP

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protease	10 (mg/ml)
2	1	reservoir	Tris-HCl	100 (mM)
3	1	reservoir	PEG4000	15 (%)