1QAQ

THE STRUCTURE OF THE RRNA METHYLTRANSFERASE ERMC': IMPLICATIONS FOR THE REACTION MECHANISM

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	100
Detector technology	CCD
Collection date	1997-11-24
Detector	MARRESEARCH
Spacegroup name	P 43 21 2
Unit cell lengths	80.930, 80.930, 120.630
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 2.800
R-factor	0.229 *
Rwork	0.229
R-free	0.28400
RMSD bond length	0.008
RMSD bond angle	1.400
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.900
High resolution limit [Å]	2.800	2.800
Rmerge	0.067	0.331
Total number of observations	40139 *
Number of reflections	10295
<I/σ(I)>	11
Completeness [%]	99.0 *	100
Redundancy	3.9	3.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5 *	298	PEG 8000, ammonium acetate , pH 7.8 at 298 K, VAPOR DIFFUSION, HANGING DROP

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	5-8 (mg/ml)
2	1	drop	Tris-HCl	25 (mM)
3	1	drop		100 (mM)
4	1	drop	dithiothreitol	2 (mM)
5	1	drop	glycerol	10 (%(v/v))
6	1	reservoir	Tes	100 (mM)
7	1	reservoir	ammonium acetate	500 (mM)
8	1	reservoir	PEG8000	15 (%(w/v))