1QAQ
THE STRUCTURE OF THE RRNA METHYLTRANSFERASE ERMC': IMPLICATIONS FOR THE REACTION MECHANISM
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1997-11-24 |
| Detector | MARRESEARCH |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 80.930, 80.930, 120.630 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.800 |
| R-factor | 0.229 * |
| Rwork | 0.229 |
| R-free | 0.28400 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.400 |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.067 | 0.331 |
| Total number of observations | 40139 * | |
| Number of reflections | 10295 | |
| <I/σ(I)> | 11 | |
| Completeness [%] | 99.0 * | 100 |
| Redundancy | 3.9 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 * | 298 | PEG 8000, ammonium acetate , pH 7.8 at 298 K, VAPOR DIFFUSION, HANGING DROP |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 5-8 (mg/ml) | |
| 2 | 1 | drop | Tris-HCl | 25 (mM) | |
| 3 | 1 | drop | 100 (mM) | ||
| 4 | 1 | drop | dithiothreitol | 2 (mM) | |
| 5 | 1 | drop | glycerol | 10 (%(v/v)) | |
| 6 | 1 | reservoir | Tes | 100 (mM) | |
| 7 | 1 | reservoir | ammonium acetate | 500 (mM) | |
| 8 | 1 | reservoir | PEG8000 | 15 (%(w/v)) |
