1QAB
The structure of human retinol binding protein with its carrier protein transthyretin reveals interaction with the carboxy terminus of RBP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE A1 |
| Synchrotron site | CHESS |
| Beamline | A1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1996-05-15 |
| Detector | ADSC |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 140.230, 140.230, 124.080 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 6.200 - 3.200 |
| R-factor | 0.278 * |
| Rwork | 0.278 |
| R-free | 0.40300 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.800 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (0.4) |
Data quality characteristics
| Overall | |
| High resolution limit [Å] | 3.200 |
| Rmerge | 0.117 |
| Total number of observations | 331570 * |
| Number of reflections | 26640 |
| Completeness [%] | 99.9 |
| Redundancy | 12.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 293 | pH 7.4, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | Hepes | ||
| 2 | 1 | 1 | Li2SO4 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 17 (mg/ml) | |
| 2 | 1 | reservoir | 1.8 (M) | ||
| 3 | 1 | reservoir | HEPES | 40 (mM) |
