1QA9
Structure of a Heterophilic Adhesion Complex Between the Human CD2 and CD58(LFA-3) Counter-Receptors
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU300 |
Temperature [K] | 298 |
Detector technology | IMAGE PLATE |
Collection date | 1998-11-12 |
Detector | MARRESEARCH |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 179.820, 33.470, 108.430 |
Unit cell angles | 90.00, 121.76, 90.00 |
Refinement procedure
Resolution | 18.000 - 3.200 |
R-factor | 0.223 * |
Rwork | 0.223 |
R-free | 0.28100 |
RMSD bond length | 0.006 |
RMSD bond angle | 1.102 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | X-PLOR (3.851) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 18.000 | 3.310 |
High resolution limit [Å] | 3.200 | 3.200 |
Rmerge | 0.102 | 0.278 |
Total number of observations | 41649 * | |
Number of reflections | 8196 | |
<I/σ(I)> | 9.8 | |
Completeness [%] | 86.7 | 87.6 |
Redundancy | 5.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | PEG 400, (NH4)2SO4, NaCl, HEPES, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 12 (mg/ml) | |
2 | 1 | drop | HEPES | 0.1 (M) | |
3 | 1 | reservoir | PEG400 | 2 (%) | |
4 | 1 | reservoir | ammonium sulfate | 2 (M) | |
5 | 1 | reservoir | 1 (M) | ||
6 | 1 | reservoir | HEPES | 0.1 (M) |