1QA9

Structure of a Heterophilic Adhesion Complex Between the Human CD2 and CD58(LFA-3) Counter-Receptors

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU300
Temperature [K]	298
Detector technology	IMAGE PLATE
Collection date	1998-11-12
Detector	MARRESEARCH
Spacegroup name	C 1 2 1
Unit cell lengths	179.820, 33.470, 108.430
Unit cell angles	90.00, 121.76, 90.00

Refinement procedure

Resolution	18.000 - 3.200
R-factor	0.223 *
Rwork	0.223
R-free	0.28100
RMSD bond length	0.006
RMSD bond angle	1.102
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	X-PLOR (3.851)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	18.000	3.310
High resolution limit [Å]	3.200	3.200
Rmerge	0.102	0.278
Total number of observations	41649 *
Number of reflections	8196
<I/σ(I)>	9.8
Completeness [%]	86.7	87.6
Redundancy	5.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	298	PEG 400, (NH4)2SO4, NaCl, HEPES, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	12 (mg/ml)
2	1	drop	HEPES	0.1 (M)
3	1	reservoir	PEG400	2 (%)
4	1	reservoir	ammonium sulfate	2 (M)
5	1	reservoir		1 (M)
6	1	reservoir	HEPES	0.1 (M)