1QA0

BOVINE TRYPSIN 2-AMINOBENZIMIDAZOLE COMPLEX

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU300
Temperature [K]	298
Detector technology	IMAGE PLATE
Collection date	1994-10-10
Detector	RIGAKU RAXIS II
Spacegroup name	P 31 2 1
Unit cell lengths	55.020, 55.020, 109.140
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	10.000 - 1.800
R-factor	0.172
Rwork	0.172
RMSD bond length	0.017
RMSD bond angle	0.033
Data scaling software	SCALEPACK
Refinement software	PROFFT

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	10.000	1.900
High resolution limit [Å]	1.800	1.800
Rmerge	0.095	0.026 *
Number of reflections	16893
<I/σ(I)>	8.1
Completeness [%]	91.1	78.5
Redundancy	6.3	4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.4	298	MICROCRYSTALS WERE INITIALLY GROWN USING THE HANGING DROP METHOD IN LINBRO CULTURE PLATES. CRYSTALLIZATION RESERVOIRS CONTAIN 1.8 - 2.0 M AMMONIUM SULFATE, 50 MM TRIS HCL, 10 MM CACL2, PH 7.4. SIX UL OF 15 MG/ML BOVINE TRYPSIN IN 20 MM 2-AMINOBENZIMIDAZOLE WAS ADDED TO 6 UL OF RESERVOIR. MACROSEEDING WAS USED TO GROW LARGE CRYSTALS (REF 2). SINGLE CRYSTALS WERE WASHED IN 0.8 M AMMONIUM SULFATE, 50 MM TRIS HCL, 10 MM CACL2, PH 7.4. THE WASHED CRYSTALS WERE PLACED IN A 18 HOUR OLD HANGING., VAPOR DIFFUSION, HANGING DROP, temperature 298K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	ammonium sulfate	1.8-2.0 (M)
2	1	reservoir	Tris-HCl	50 (mM)
3	1	reservoir		10 (mM)
4	1	drop	trypsin	15 (mg/ml)
5	1	drop	2-aminobenzimidazole	20 (mM)