1Q9X
Crystal structure of Enterobacteria phage RB69 gp43 DNA polymerase complexed with tetrahydrofuran containing DNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X26C |
Synchrotron site | NSLS |
Beamline | X26C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2002-09-10 |
Detector | ADSC QUANTUM 4 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 131.948, 122.237, 165.358 |
Unit cell angles | 90.00, 96.85, 90.00 |
Refinement procedure
Resolution | 47.000 - 2.690 |
R-factor | 0.21879 |
Rwork | 0.215 |
R-free | 0.28790 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1clq |
RMSD bond length | 0.013 |
RMSD bond angle | 1.535 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | REFMAC (5.1.24) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.000 | 2.800 |
High resolution limit [Å] | 2.690 | 2.690 |
Number of reflections | 137338 | |
<I/σ(I)> | 19.1 | 1.8 |
Completeness [%] | 94.7 | 73.3 |
Redundancy | 4.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 6.5 | 298 | PEG 350MME, Calcium chloride, Tris-HCl, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K, pH 6.50 |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | PEG 350 | ||
2 | 1 | 1 | Calcium chloride | ||
3 | 1 | 1 | Tris-HCl | ||
4 | 1 | 1 | H2O | ||
5 | 1 | 2 | PEG 350 | ||
6 | 1 | 2 | Calcium chloride | ||
7 | 1 | 2 | Tris-HCl | ||
8 | 1 | 2 | H2O |