1Q9U

Crystal structure of uncharacterized conserved protein DUF302 from Bacillus stearothermophilus

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2003-08-06
Detector	SBC-2
Wavelength(s)	1.28322, 0.97932
Spacegroup name	P 21 21 21
Unit cell lengths	43.185, 47.268, 110.087
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.430 - 1.800
R-factor	0.192
Rwork	0.192
R-free	0.24100
Structure solution method	autoSHARP/wARP
RMSD bond length	0.007
RMSD bond angle	1.300
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SHARP
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.860
High resolution limit [Å]	1.800	1.800
Rmerge	0.052
Number of reflections	21413
<I/σ(I)>	6.6
Completeness [%]	98.8	92.7
Redundancy	5.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	298	1,4,-butanedial, imidazole, Zn(OAc)2, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 298K