1Q9H

3-Dimensional structure of native Cel7A from Talaromyces emersonii

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE BW7B
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	BW7B
Temperature [K]	298
Detector technology	AREA DETECTOR
Collection date	1996-01-01
Detector	MARRESEARCH
Wavelength(s)	0.8384
Spacegroup name	P 41 21 2
Unit cell lengths	74.395, 74.395, 177.046
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.650 - 2.350
R-factor	0.16462
Rwork	0.161
R-free	0.22934
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.012
RMSD bond angle	1.492
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	REFMAC (5.1.24)

Data quality characteristics

	Overall
Low resolution limit [Å]	19.650
High resolution limit [Å]	2.350
Number of reflections	20984

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	291	2.5 M ammonium phosphate, 100 mM Tris-HCl, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K