1Q9C
Crystal Structure of the Histone domain of Son of Sevenless
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.2 |
Synchrotron site | ALS |
Beamline | 8.2.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2002-09-26 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.9793 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 93.397, 109.199, 212.399 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 * - 3.210 |
Rwork | 0.255 |
R-free | 0.29200 |
Structure solution method | SAD |
RMSD bond length | 0.003 |
RMSD bond angle | 18.300 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 99.000 * | 3.310 |
High resolution limit [Å] | 3.200 * | 3.210 |
Rmerge | 0.066 * | 0.365 * |
Number of reflections | 35294 | |
<I/σ(I)> | 20.1 | 4 |
Completeness [%] | 99.0 * | 96.9 * |
Redundancy | 7.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | PEG3350, L-proline, magnesium acetate, ethanol, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 5-20 (mg/ml) | |
2 | 1 | reservoir | PEG3350 | 4-12 (%) | |
3 | 1 | reservoir | L-proline | 0.2 (M) | |
4 | 1 | reservoir | magnesium acetate | 0.1 (M) | |
5 | 1 | reservoir | ethanol | 5 (%) |