1Q99

Crystal structure of the Saccharomyces cerevisiae SR protein kinsae, Sky1p, complexed with the non-hydrolyzable ATP analogue, AMP-PNP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Detector technology	CCD
Collection date	2001-11-15
Detector	SBC-2
Wavelength(s)	1.54
Spacegroup name	P 21 21 21
Unit cell lengths	72.413, 88.947, 134.822
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.950 - 2.100 *
Rwork	0.220
R-free	0.26010 *
RMSD bond length	0.007 *
RMSD bond angle	1.270 *
Data reduction software	HKL-2000
Data scaling software	SORTRF
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.110	2.180
High resolution limit [Å]	2.100 *	2.110
Rmerge	0.085 *	46.700 *
Total number of observations	280589 *
Number of reflections	49644 *
Completeness [%]	96.5 *	86.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.5	298	Nolen, B., (2001) Nat.Struct.Biol., 8, 176. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	15 (mg/ml)
2	1	drop	ATP	0.0066 (mM)
3	1	drop		0.0017 (mM)
4	1	reservoir	ammonium sulfate	1.5 (M)
5	1	reservoir	ethylene glycol	15 (%(v/v))
6	1	reservoir	sodium acetate	10 (mM)