1Q94
Structures of HLA-A*1101 in complex with immunodominant nonamer and decamer HIV-1 epitopes clearly reveal the presence of a middle anchor residue
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F1 |
| Synchrotron site | CHESS |
| Beamline | F1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-06-01 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9474 |
| Spacegroup name | P 1 |
| Unit cell lengths | 45.400, 46.400, 124.300 |
| Unit cell angles | 91.50, 97.90, 114.80 |
Refinement procedure
| Resolution | 23.700 - 2.400 |
| R-factor | 0.234 |
| Rwork | 0.232 |
| R-free | 0.27200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.400 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 24.000 | 2.340 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.124 | 0.158 |
| Number of reflections | 36125 | |
| <I/σ(I)> | 17.9 | 7.3 |
| Completeness [%] | 89.5 | 40.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | PEG 4000,sodium acetate,sodium citrate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 100K | |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | PEG 4000,sodium acetate,sodium citrate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 100K |
