1Q93
Crystal structure of a mutant of the sarcin/ricin domain from rat 28S rRNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-BM-C |
| Synchrotron site | APS |
| Beamline | 14-BM-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-03-08 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 43 2 2 |
| Unit cell lengths | 42.143, 42.143, 336.167 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.000 - 2.250 |
| R-factor | 0.227 |
| Rwork | 0.225 |
| R-free | 0.26600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 483d |
| RMSD bond length | 0.004 * |
| RMSD bond angle | 0.800 * |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | EPMR |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.290 |
| High resolution limit [Å] | 2.250 | 2.250 |
| Rmerge | 0.077 | 0.473 |
| Number of reflections | 15569 | |
| <I/σ(I)> | 41.1 | 6.8 |
| Completeness [%] | 98.8 * | 98 |
| Redundancy | 9.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 7 | 295 | ammonium sulfate, K-MOPS, magnesium chloride, spermine, cobalt chloride, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | ammonium sulfate | ||
| 10 | 1 | 2 | K-MOPS | ||
| 11 | 1 | 2 | H2O | ||
| 2 | 1 | 1 | K-MOPS | ||
| 3 | 1 | 1 | magnesium chloride | ||
| 4 | 1 | 1 | spermine | ||
| 5 | 1 | 1 | cobalt chloride | ||
| 6 | 1 | 1 | H2O | ||
| 7 | 1 | 2 | ammonium sulfate | ||
| 8 | 1 | 2 | spermine | ||
| 9 | 1 | 2 | cobalt chloride |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | RNA | 2.5 (mg/ml) | |
| 2 | 1 | drop | potassium MOPS | 50 (mM) | pH7.0 |
| 3 | 1 | drop | 5 (mM) | ||
| 4 | 1 | reservoir | ammonium sulfate | 3.0-3.2 (M) | |
| 5 | 1 | reservoir | potassium MOPS | 50 (mM) | pH7.0 |
| 6 | 1 | reservoir | 20 (mM) | ||
| 7 | 1 | reservoir | spermine | 1 (mM) | |
| 8 | 1 | reservoir | 2 (mM) |
