1Q8Y

The structure of the yeast SR protein kinase, Sky1p, with bound ADP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	94
Detector technology	CCD
Collection date	2001-11-15
Detector	SBC-2
Wavelength(s)	1.54
Spacegroup name	P 21 21 21
Unit cell lengths	71.577, 88.653, 133.786
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.920 - 2.050
Rwork	0.209
R-free	0.24460 *
RMSD bond length	0.006
RMSD bond angle	1.260 *
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.840	2.150
High resolution limit [Å]	2.050	2.050
Rmerge	0.068 *	0.411 *
Total number of observations	298198 *
Number of reflections	50520 *
Completeness [%]	98.1	93.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.5	298	Nolen, B., (2001) Nat.Struct.Biol., 8, 176. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	15 (mg/ml)
2	1	drop	ATP	0.0066 (mM)
3	1	drop		0.0017 (mM)
4	1	reservoir	ammonium sulfate	1.5 (M)
5	1	reservoir	ethylene glycol	15 (%(v/v))
6	1	reservoir	sodium acetate	10 (mM)