1Q8R
Structure of E.coli RusA Holliday junction resolvase
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-04-25 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.97943 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 45.352, 50.052, 49.597 |
| Unit cell angles | 90.00, 101.42, 90.00 |
Refinement procedure
| Resolution | 19.200 * - 1.900* |
| R-factor | 0.19199 |
| Rwork | 0.190 |
| R-free | 0.22600 * |
| Structure solution method | MAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.014 * |
| RMSD bond angle | 2.800 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHELXD |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.950 |
| High resolution limit [Å] | 1.900 * | 1.900 * |
| Rmerge | 0.028 | 0.103 |
| Number of reflections | 17267 | |
| <I/σ(I)> | 12.25 | 8.58 |
| Completeness [%] | 99.0 * | 97 |
| Redundancy | 3.7 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 290 | PEG 4000, sodium acetate, Tris-HCl, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 290K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 5 (mg/ml) | |
| 2 | 1 | reservoir | PEG4000 | 30 (%) | |
| 3 | 1 | reservoir | sodium acetate | 0.3 (M) | in Tris-HCl, pH8 |
