1Q8R

Structure of E.coli RusA Holliday junction resolvase

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-4
Synchrotron site	ESRF
Beamline	ID14-4
Temperature [K]	100
Detector technology	CCD
Collection date	2001-04-25
Detector	ADSC QUANTUM 4
Wavelength(s)	0.97943
Spacegroup name	P 1 21 1
Unit cell lengths	45.352, 50.052, 49.597
Unit cell angles	90.00, 101.42, 90.00

Refinement procedure

Resolution	19.200 * - 1.900 *
R-factor	0.19199
Rwork	0.190
R-free	0.22600 *
Structure solution method	MAD
Starting model (for MR)	NONE
RMSD bond length	0.014 *
RMSD bond angle	2.800 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHELXD
Refinement software	REFMAC (5.1.24)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.950
High resolution limit [Å]	1.900 *	1.900 *
Rmerge	0.028	0.103
Number of reflections	17267
<I/σ(I)>	12.25	8.58
Completeness [%]	99.0 *	97
Redundancy	3.7	3.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	290	PEG 4000, sodium acetate, Tris-HCl, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 290K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	5 (mg/ml)
2	1	reservoir	PEG4000	30 (%)
3	1	reservoir	sodium acetate	0.3 (M)	in Tris-HCl, pH8