1Q8A
Cobalamin-dependent methionine synthase (1-566) from Thermotoga maritima (Cd2+:L-Hcy complex, Se-Met)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 5ID-B |
| Synchrotron site | APS |
| Beamline | 5ID-B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-03-12 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 59.120, 86.190, 126.080 |
| Unit cell angles | 90.00, 99.99, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.650 * |
| R-factor | 0.225 |
| Rwork | 0.214 |
| R-free | 0.23900 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.013 * |
| RMSD bond angle | 1.687 * |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.780 |
| High resolution limit [Å] | 1.650 * | 1.700 |
| Rmerge | 0.064 * | 0.408 * |
| Number of reflections | 147923 * | |
| Completeness [%] | 99.4 * | 99.8 * |
| Redundancy | 5.8 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.7 | 4 * | propylene glycol, glycerol, PEG 8000, citrate, 1,2,3-heptanetriol, cadmium, pH 5.7, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | 10 (mM) | ||
| 2 | 1 | drop | 1,2,3-heptanetriol | 1.25 (%(w/v)) | |
| 3 | 1 | reservoir | 1,2-propanediol | 25 (%) | |
| 4 | 1 | reservoir | glycerol | 10 (%) | |
| 5 | 1 | reservoir | PEG3000 | 5 (%) | |
| 6 | 1 | reservoir | sodium citrate | 100 (mM) | pH5.30 |
