1Q87
Crystal structure of the C-domain of the T.vaginalis Inr binding protein, IBP39 (tetragonal form)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-11-12 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.08 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 76.420, 76.420, 157.620 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 65.000 - 2.320 |
| R-factor | 0.215 |
| Rwork | 0.215 |
| R-free | 0.27500 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1q88 C-domain from the C2 monoclinic form |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.500 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | MOLREP |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 65.000 | 2.380 |
| High resolution limit [Å] | 2.320 | 2.320 |
| Rmerge | 0.042 | 0.386 |
| Total number of observations | 111463 * | |
| Number of reflections | 19164 | |
| <I/σ(I)> | 2 | |
| Completeness [%] | 91.6 | 91.6 |
| Redundancy | 5.8 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 6.5 | 298 | PEG 5000, ammonium sulphate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 20-50 (mg/ml) | |
| 2 | 1 | reservoir | ammonium sulfate | 200 (mM) | |
| 3 | 1 | reservoir | MES | 50 (mM) | pH6.5 |
| 4 | 1 | reservoir | PEG5000 | 25 (%) |
