1Q7T
Rv1170 (MshB) from Mycobacterium tuberculosis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-04-27 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 71.690, 83.740, 95.650 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 * - 1.900 |
| R-factor | 0.19 |
| Rwork | 0.190 |
| R-free | 0.21400 * |
| Structure solution method | MIR |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.220 * |
| Data reduction software | DENZO |
| Data scaling software | XDS |
| Phasing software | SHARP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.065 * | 0.216 * |
| Total number of observations | 324835 * | |
| Number of reflections | 44945 * | |
| <I/σ(I)> | 17.83 | |
| Completeness [%] | 97.6 * | 94.3 * |
| Redundancy | 7.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 * | 291 | McCarthy, A.A., (2003) Acta Cryst., D59, 2316. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | PEG2000 MME | 24-27 (%) | |
| 2 | 1 | reservoir | ammonium sulfate | 0.2 (M) | |
| 3 | 1 | reservoir | sodium cacodylate | 100 (mM) | pH6.1 |
| 4 | 1 | drop | protein | 10 (mg/ml) | |
| 5 | 1 | drop | HEPES | 10 (mM) | pH7.5 |
