1Q7H

Structure of a Conserved PUA Domain Protein from Thermoplasma acidophilum

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	150
Detector technology	CCD
Collection date	2003-08-02
Detector	CUSTOM-MADE
Wavelength(s)	0.97967, 0.97951, 0.95667
Spacegroup name	C 2 2 21
Unit cell lengths	48.228, 58.158, 112.218
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 2.100
R-factor	0.171
Rwork	0.169
R-free	0.22800
Structure solution method	MAD
RMSD bond length	0.007
RMSD bond angle	1.500
Data reduction software	HKL-2000
Data scaling software	d*TREK
Phasing software	SOLVE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.180
High resolution limit [Å]	2.100	2.100
Number of reflections	8365
<I/σ(I)>	17.3	2.7
Completeness [%]	87.8	55.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.4	294	Tris, Magnesium Formate, Glycerol, pH 8.4, VAPOR DIFFUSION, HANGING DROP, temperature 294K