1Q7G
Homoserine Dehydrogenase in complex with suicide inhibitor complex NAD-5-hydroxy-4-Oxonorvaline
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X8C |
| Synchrotron site | NSLS |
| Beamline | X8C |
| Temperature [K] | 218 |
| Detector technology | CCD |
| Collection date | 2001-02-28 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.0722 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 80.863, 80.863, 248.986 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.430 - 2.600 |
| R-factor | 0.247 |
| Rwork | 0.247 |
| R-free | 0.29500 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ebf |
| RMSD bond length | 0.007 * |
| RMSD bond angle | 22.800 * |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 99.000 | 2.690 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.081 | 0.257 |
| Number of reflections | 24854 | |
| Completeness [%] | 94.6 | 72 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.4 | 295 | DeLaBarre, B., (1998) Acta Crystallogr., Sect.D, 54, 413. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | ammonium sulfate | 2.2 (M) | |
| 2 | 1 | reservoir | sodium acetate | 0.1 (M) | pH4.4 |
| 3 | 1 | drop | protein | 10 (mg/ml) | |
| 4 | 1 | drop | Tris-HCl | 10 (mM) | or 10 mM HEPES |
| 5 | 1 | drop | 20 (mM) |
