1Q77

X-ray crystal structure of putative Universal Stress Protein from Aquifex aeolicus

Experimental procedure

Experimental method	SAD
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2003-07-20
Detector	SBC-2
Wavelength(s)	0.97962
Spacegroup name	P 43 21 2
Unit cell lengths	94.375, 94.375, 134.542
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.380 - 2.700
R-factor	0.22
Rwork	0.220
R-free	0.25700
Structure solution method	SAD
RMSD bond length	0.007
RMSD bond angle	1.300
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.800
High resolution limit [Å]	2.700	2.700
Rmerge	0.104
Number of reflections	17349
<I/σ(I)>	6.1
Completeness [%]	99.5	95.1
Redundancy	14.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	9	289	ammonium sulfate, Tris buffer, pH 9, VAPOR DIFFUSION, HANGING DROP, temperature 289K