1Q6Y
Hypothetical protein YfdW from E. coli bound to Coenzyme A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-06-27 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.9793 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 92.117, 66.779, 73.049 |
| Unit cell angles | 90.00, 108.73, 90.00 |
Refinement procedure
| Resolution | 19.780 - 1.990 |
| Rwork | 0.160 |
| R-free | 0.20000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1pqy |
| RMSD bond length | 0.020 |
| RMSD bond angle | 2.000 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.070 |
| High resolution limit [Å] | 1.990 | 2.000 |
| Number of reflections | 27082 | |
| <I/σ(I)> | 10.5 | 5 |
| Completeness [%] | 93.6 | 72.3 |
| Redundancy | 3.4 | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 65% MPD, 0.1 M Bis-Tris pH 6.5, 1.5 mM CoA, 1.5 mM oxalate, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
