1Q6Y
Hypothetical protein YfdW from E. coli bound to Coenzyme A
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 31-ID |
Synchrotron site | APS |
Beamline | 31-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-06-27 |
Detector | MARRESEARCH |
Wavelength(s) | 0.9793 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 92.117, 66.779, 73.049 |
Unit cell angles | 90.00, 108.73, 90.00 |
Refinement procedure
Resolution | 19.780 - 1.990 |
Rwork | 0.160 |
R-free | 0.20000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1pqy |
RMSD bond length | 0.020 |
RMSD bond angle | 2.000 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.070 |
High resolution limit [Å] | 1.990 | 2.000 |
Number of reflections | 27082 | |
<I/σ(I)> | 10.5 | 5 |
Completeness [%] | 93.6 | 72.3 |
Redundancy | 3.4 | 2.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 65% MPD, 0.1 M Bis-Tris pH 6.5, 1.5 mM CoA, 1.5 mM oxalate, VAPOR DIFFUSION, HANGING DROP, temperature 298K |