1Q67
Crystal structure of Dcp1p
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | MARRESEARCH |
| Spacegroup name | P 3 2 1 |
| Unit cell lengths | 123.550, 123.550, 77.900 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 2.300 |
| R-factor | 0.227 |
| Rwork | 0.227 |
| R-free | 0.26800 |
| Structure solution method | SAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.300 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.260 |
| High resolution limit [Å] | 2.300 * | 2.200 |
| Rmerge | 0.066 | 0.371 * |
| Total number of observations | 286716 * | |
| Number of reflections | 34251 | |
| <I/σ(I)> | 8.3 | 1.1 |
| Completeness [%] | 97.9 | 97.9 |
| Redundancy | 8.4 | 4.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.6 * | VAPOR DIFFUSION, HANGING DROP |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 12 (mg/ml) | |
| 10 | 1 | reservoir | EDTA | 6 (mM) | |
| 11 | 1 | reservoir | isopropanol | 2 (%(v/v)) | |
| 2 | 1 | drop | Tris-HCl | 20 (mM) | pH7.6 |
| 3 | 1 | drop | 100 (mM) | ||
| 4 | 1 | drop | 2 (mM) | ||
| 5 | 1 | drop | dithiothreitol | 2 (mM) | |
| 6 | 1 | reservoir | ammonium sulfate | 0.76 (M) | |
| 7 | 1 | reservoir | 1.50 (M) | ||
| 8 | 1 | reservoir | dithiothreitol | 5 (mM) | |
| 9 | 1 | reservoir | Tris-HCl | 0.1 (M) | pH8.5 |
