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1Q67

Crystal structure of Dcp1p

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsSPRING-8 BEAMLINE BL41XU
Synchrotron siteSPring-8
BeamlineBL41XU
Temperature [K]100
Detector technologyCCD
DetectorMARRESEARCH
Spacegroup nameP 3 2 1
Unit cell lengths123.550, 123.550, 77.900
Unit cell angles90.00, 90.00, 120.00
Refinement procedure
Resolution20.000 - 2.300
R-factor0.227
Rwork0.227
R-free0.26800
Structure solution methodSAD
RMSD bond length0.006
RMSD bond angle1.300
Data reduction softwareDENZO
Data scaling softwareSCALEPACK
Phasing softwareSHARP
Refinement softwareCNS (1.0)
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]50.0002.260
High resolution limit [Å]2.300

*

2.200
Rmerge0.0660.371

*

Total number of observations286716

*

Number of reflections34251
<I/σ(I)>8.31.1
Completeness [%]97.997.9
Redundancy8.44.9
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1Vapor diffusion, hanging drop

*

7.6

*

VAPOR DIFFUSION, HANGING DROP
Crystallization Reagents in Literatures
IDcrystal IDsolutionreagent nameconcentration (unit)details
11dropprotein12 (mg/ml)
101reservoirEDTA6 (mM)
111reservoirisopropanol2 (%(v/v))
21dropTris-HCl20 (mM)pH7.6
31drop100 (mM)
41drop2 (mM)
51dropdithiothreitol2 (mM)
61reservoirammonium sulfate0.76 (M)
71reservoir1.50 (M)
81reservoirdithiothreitol5 (mM)
91reservoirTris-HCl0.1 (M)pH8.5

217705

PDB entries from 2024-03-27

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