1Q5Z
Crystal Structure of the C-terminal Actin Binding Domain of Salmonella Invasion Protein A (SipA)
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X9A |
| Synchrotron site | NSLS |
| Beamline | X9A |
| Temperature [K] | 113.15 |
| Detector technology | CCD |
| Collection date | 2002-09-18 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.9792,0.9794,0.9716 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 79.900, 79.900, 57.500 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.800 |
| R-factor | 0.194 |
| Rwork | 0.194 |
| R-free | 0.22200 |
| Structure solution method | MAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.260 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.060 * | 0.426 * |
| Total number of observations | 476002 * | |
| Number of reflections | 17843 * | |
| Completeness [%] | 99.9 * | 100 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 * | 277 | 2M ammonium sulfate, 1.5% methyl-pentanediol, 0.1M Hepes, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 40 (mg/ml) | |
| 2 | 1 | drop | Tris | 10 (mM) | pH8.0 |
| 3 | 1 | drop | 100 (mM) | ||
| 4 | 1 | drop | dithiothreitol | 2 (mM) | |
| 5 | 1 | reservoir | ammonium sulfate | 2 (M) | |
| 6 | 1 | reservoir | MPD | 1.5 (%) | pH7.5 |
| 7 | 1 | reservoir | dithiothreitol | 2 (mM) |
