1Q4R
Gene Product of At3g17210 from Arabidopsis Thaliana
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 14-ID-B |
Synchrotron site | APS |
Beamline | 14-ID-B |
Temperature [K] | 90 |
Detector technology | CCD |
Collection date | 2003-06-26 |
Detector | MARRESEARCH |
Wavelength(s) | 0.979 |
Spacegroup name | P 62 |
Unit cell lengths | 55.468, 55.468, 57.673 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 20.000 - 1.900 |
R-factor | 0.18469 |
Rwork | 0.182 |
R-free | 0.23200 * |
Structure solution method | MAD |
RMSD bond length | 0.020 |
RMSD bond angle | 1.861 |
Data scaling software | SCALEPACK |
Phasing software | SOLVE (RESOLVE) |
Refinement software | REFMAC (5.1.24) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 1.970 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.042 | 0.274 * |
Total number of observations | 75176 * | |
Number of reflections | 14162 * | |
<I/σ(I)> | 35.6 | 2.06 |
Completeness [%] | 93.5 * | 52.6 * |
Redundancy | 5.2 * | 2.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 * | 297 | Sodium MES, Sodium citrate, MEthyl Ether PEG 5000, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 297K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | mPEG5000 | 30 (%(w/v)) | |
2 | 1 | reservoir | sodium cirtate | 120 (mM) | |
3 | 1 | reservoir | sodium HEPES | 100 (mM) | pH6.0 |
4 | 1 | drop | 100 (mM) | ||
5 | 1 | drop | sodium HEPES | 10 (mM) | pH7.0 |
6 | 1 | drop | protein | 14 (mg/ml) |