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1Q4D

S65T Q80R T203C Green Fluorescent Protein (GFP) pH 5.5

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsSSRL BEAMLINE BL7-1
Synchrotron siteSSRL
BeamlineBL7-1
Spacegroup nameP 21 21 21
Unit cell lengths50.969, 62.333, 68.554
Unit cell angles90.00, 90.00, 90.00
Refinement procedure
Resolution25.000 - 1.580
R-factor0.208
Rwork0.208
R-free0.23300
Structure solution methodMOLECULAR REPLACEMENT
Starting model (for MR)1ema
RMSD bond length0.010

*

RMSD bond angle1.780

*

Data reduction softwareDENZO
Data scaling softwareSCALEPACK
Phasing softwareCNS
Refinement softwareCNS
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]25.000
High resolution limit [Å]1.580
Rmerge0.046

*

0.436

*

Number of reflections30578
Completeness [%]99.999.4

*

Redundancy4.7

*

Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, HANGING DROP5.5298PEG 4000, MgCl2, BME, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K
Crystallization Reagents in Literatures
IDcrystal IDsolutionreagent nameconcentration (unit)details
11reservoirPEG400022-26 (%)
21reservoir50 (mM)
31reservoir2-mercaptoethanol10 (mM)
41reservoirHEPES50 (mM)pH8.1-8.5
51dropprotein12-15 (mg/ml)
61dropHEPES50 (mM)pH7.5

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PDB entries from 2024-09-18

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