1Q4C
S65T Q80R T203C Green Fluorescent Protein (GFP) pH 8.5
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 50.880, 62.430, 68.906 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.000 - 1.550 |
| R-factor | 0.194 |
| Rwork | 0.194 |
| R-free | 0.21400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ema |
| RMSD bond length | 0.010 * |
| RMSD bond angle | 1.670 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 24.000 * | |
| High resolution limit [Å] | 1.550 | |
| Rmerge | 0.057 * | 0.423 * |
| Number of reflections | 31021 | |
| Completeness [%] | 95.3 | 92.4 * |
| Redundancy | 3.9 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 298 | PEG 4000, MgCl2, BME, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | PEG4000 | 22-26 (%) | |
| 2 | 1 | reservoir | 50 (mM) | ||
| 3 | 1 | reservoir | 2-mercaptoethanol | 10 (mM) | |
| 4 | 1 | reservoir | HEPES | 50 (mM) | pH8.1-8.5 |
| 5 | 1 | drop | protein | 12-15 (mg/ml) | |
| 6 | 1 | drop | HEPES | 50 (mM) | pH7.5 |
