1Q4A
S65T Q80R Green Fluorescent Protein (GFP) pH 8.5
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Detector technology | IMAGE PLATE |
| Detector | RIGAKU RAXIS IV |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 50.864, 62.351, 69.174 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.000 - 1.450 |
| R-factor | 0.187 |
| Rwork | 0.187 |
| R-free | 0.20100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ema |
| RMSD bond length | 0.010 * |
| RMSD bond angle | 1.740 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | |
| High resolution limit [Å] | 1.450 | |
| Rmerge | 0.041 * | 0.302 * |
| Number of reflections | 36969 | |
| Completeness [%] | 93.3 | 68.5 * |
| Redundancy | 2.8 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 298 | PEG 4000, MgCl2, BME, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | PEG4000 | 22-26 (%) | |
| 2 | 1 | reservoir | 50 (mM) | ||
| 3 | 1 | reservoir | 2-mercaptoethanol | 10 (mM) | |
| 4 | 1 | reservoir | HEPES | 50 (mM) | pH8.1-8.5 |
| 5 | 1 | drop | protein | 12-15 (mg/ml) | |
| 6 | 1 | drop | HEPES | 50 (mM) | pH7.5 |
