1Q47
Structure of the Semaphorin 3A Receptor-Binding Module
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 8-BM |
| Synchrotron site | APS |
| Beamline | 8-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-02-02 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97, 0.964 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 218.791, 59.733, 122.715 |
| Unit cell angles | 90.00, 109.01, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.800 |
| R-factor | 0.249 |
| R-free | 0.29400 |
| Structure solution method | MAD |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MLPHARE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.930 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Number of reflections | 277955 | |
| Completeness [%] | 99.9 | 100 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | Tris, PEG 8000, heptyl-beta-D thioglucoside, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
