1Q46
crystal structure of the eIF2 alpha subunit from saccharomyces cerevisia
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 293 |
Detector technology | IMAGE PLATE |
Collection date | 2003-03-20 |
Detector | MARRESEARCH |
Wavelength(s) | 1.54 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 94.009, 94.009, 81.198 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.860 |
R-factor | 0.222 |
Rwork | 0.218 |
R-free | 0.24300 * |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | human eIF2 alpha subunit |
RMSD bond length | 0.006 * |
RMSD bond angle | 1.210 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CCP4 |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.960 |
High resolution limit [Å] | 2.860 | 2.860 |
Rmerge | 0.077 * | 0.583 * |
Total number of observations | 77383 * | |
Number of reflections | 9935 * | |
<I/σ(I)> | 1.3 | |
Completeness [%] | 99.5 * | 99.9 |
Redundancy | 7.78 * |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | unknown * | 4.6 | 293 | 0.1 M sodium acetate (pH=4.6), 1.8-2.5 M sodium formate, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | 1 | sodium formate | 1.8-2.5 (M) | |
2 | 1 | 1 | acetate | 0.1 (M) |