1Q3Q
Crystal structure of the chaperonin from Thermococcus strain KS-1 (two-point mutant complexed with AMP-PNP)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL45XU |
| Synchrotron site | SPring-8 |
| Beamline | BL45XU |
| Temperature [K] | 90 |
| Detector technology | IMAGE PLATE |
| Detector | RIGAKU RAXIS |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 4 21 2 |
| Unit cell lengths | 210.829, 210.829, 156.421 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 41.900 * - 2.300 |
| R-factor | 0.213 |
| Rwork | 0.213 |
| R-free | 0.25000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1q2v |
| RMSD bond length | 0.006 |
| RMSD bond angle | 21.400 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 * | |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.117 * | 0.304 * |
| Total number of observations | 590450 * | |
| Number of reflections | 148110 | |
| <I/σ(I)> | 12.4 | 2.6 |
| Completeness [%] | 96.2 | 89.7 |
| Redundancy | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 8 | 20 * | ammonium sulfate, potassium chloride, Tris, pH 8, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 15-20 (mg/ml) | |
| 2 | 1 | reservoir | Tris | 100 (mM) | pH8.0 |
| 3 | 1 | reservoir | ammonium sulfate | 2.1 (M) | |
| 4 | 1 | reservoir | 50 (mM) |
