1Q3H
mouse CFTR NBD1 with AMP.PNP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 31-ID |
Synchrotron site | APS |
Beamline | 31-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-03-12 |
Detector | MARRESEARCH |
Wavelength(s) | 0.9198 |
Spacegroup name | P 4 21 2 |
Unit cell lengths | 171.680, 171.680, 109.590 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 33.000 - 2.500 |
R-factor | 0.218 |
Rwork | 0.216 |
R-free | 0.26600 * |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.008 * |
RMSD bond angle | 1.900 * |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 33.000 | 2.630 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.122 * | 0.350 * |
Number of reflections | 57029 | |
Completeness [%] | 99.9 | 99.6 * |
Redundancy | 14.1 * | 13.6 * |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 4, 20 * | Sodium acetate, tris buffer, sodium chloride, DTT, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277.0K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 5-10 (mg/ml) | |
2 | 1 | reservoir | sodium acetate | 3.5-4.0 (M) |