1Q33
Crystal structure of human ADP-ribose pyrophosphatase NUDT9
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 170 |
| Detector technology | CCD |
| Collection date | 2002-03-21 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.997 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 79.837, 87.497, 119.814 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 36.540 - 1.810 |
| R-factor | 0.2037 |
| Rwork | 0.201 |
| R-free | 0.23130 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.450 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.760 | 1.830 |
| High resolution limit [Å] | 1.810 | 1.800 |
| Number of reflections | 36910 | |
| <I/σ(I)> | 45.8 | 5.54 |
| Completeness [%] | 94.1 | 66.2 |
| Redundancy | 9.3 | 2.98 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 298 | Ammonium sulfate, TrisHCl, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
