1Q2Y
Crystal structure of the protein YJCF from Bacillus subtilis: a member of the GCN5-related N-acetyltransferase superfamily fold
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X9A |
| Synchrotron site | NSLS |
| Beamline | X9A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-02-24 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.0088 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 35.266, 59.283, 71.556 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 15.000 - 2.000 |
| R-factor | 0.239 |
| Rwork | 0.234 |
| R-free | 0.26500 |
| Structure solution method | MIR |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.360 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.000 | 2.090 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.048 | 0.153 |
| Number of reflections | 9584 | |
| <I/σ(I)> | 32.6 | 7.8 |
| Completeness [%] | 93.0 | 92.1 |
| Redundancy | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 277 | PEG 550, Ammonium tartrate, BisTris , pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
