1Q2V

Crystal structure of the chaperonin from Thermococcus strain KS-1 (nucleotide-free form)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL41XU
Synchrotron site	SPring-8
Beamline	BL41XU
Temperature [K]	90
Detector technology	CCD
Collection date	2000-06-17
Detector	MARRESEARCH
Wavelength(s)	0.708
Spacegroup name	P 4 21 2
Unit cell lengths	209.272, 209.272, 156.625
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.900 * - 2.400
R-factor	0.246
Rwork	0.246
R-free	0.26900
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	21.800 *
Data scaling software	CCP4 ((SCALA))
Phasing software	X-PLOR (3.851)
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	2.530
High resolution limit [Å]	2.400	2.400
Rmerge	0.055 *	0.426 *
Total number of observations	614552 *
Number of reflections	134160 *
<I/σ(I)>	11.7	1.7
Completeness [%]	99.3	98.1
Redundancy	4.6	3.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	8	20 *	2.1 M ammonium sulfate, 50 mM potassium chloride, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	15-20 (mg/ml)
2	1	reservoir	Tris	100 (mM)	pH8.0
3	1	reservoir	ammonium sulfate	2.1 (M)
4	1	reservoir		50 (mM)