1Q2H

Phenylalanine Zipper Mediates APS Dimerization

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X12C
Synchrotron site	NSLS
Beamline	X12C
Temperature [K]	100
Detector technology	CCD
Collection date	2002-01-01
Detector	BRANDEIS - B4
Wavelength(s)	0.95000, 0.9174, 0.9211, 0.9208
Spacegroup name	P 21 21 2
Unit cell lengths	69.740, 57.480, 51.720
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 1.700
Rwork	0.250
R-free	0.27000
Structure solution method	MAD
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS (1.0)

Data quality characteristics

	Overall
Low resolution limit [Å]	30.000
High resolution limit [Å]	1.700
Number of reflections	19012

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6		0.48-0.68 M sodium iodide, 2.5 % PEG 4K, 0.09 M sodium citrate, 10 mM DTT, pH 6.0, VAPOR DIFFUSION, HANGING DROP