1Q2D
Crystal Structure of Tetrahymena GCN5 With Bound Coenzyme A and a 19-residue p53 peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE A1 |
| Synchrotron site | CHESS |
| Beamline | A1 |
| Detector technology | CCD |
| Collection date | 2000-06-08 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.9213 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 64.740, 64.740, 96.413 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 27.880 - 2.250 |
| Rwork | 0.247 |
| R-free | 0.27700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1qsn |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.515 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 27.800 |
| High resolution limit [Å] | 1.900 |
| Number of reflections | 23773 |
| Completeness [%] | 87.0 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 277 | Ammonium sulfate, Hepes, Sodium chloride, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
