1Q2C
Crystal Structure of Tetrahymena GCN5 With Bound Coenzyme A and a 19-residue Histone H4 Peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE A1 |
| Synchrotron site | CHESS |
| Beamline | A1 |
| Detector technology | CCD |
| Collection date | 2000-06-08 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.9213 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 65.132, 65.132, 96.568 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 27.070 - 2.250 |
| R-factor | 0.2521 |
| Rwork | 0.252 |
| R-free | 0.27900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.238 |
| RMSD bond angle | 5.700 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 27.000 | 2.250 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 23766 | |
| Completeness [%] | 93.5 | 94.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 298 | Ammonium sulfate, Tris-HCl, Magnesium Chloride, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
