1Q1W
Crystal Structure of Putidaredoxin Reductase from Pseudomonas putida
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 200 |
| Detector technology | IMAGE PLATE |
| Collection date | 2001-05-10 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 70.040, 104.327, 166.602 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 * - 2.600 |
| Rwork | 0.203 |
| R-free | 0.23800 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1q1r |
| RMSD bond length | 0.006 * |
| RMSD bond angle | 1.500 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.690 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.077 | 0.561 |
| Number of reflections | 37470 * | |
| <I/σ(I)> | 12 | 2 |
| Completeness [%] | 97.1 * | 91.3 * |
| Redundancy | 3.6 | 1.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 298 | lithium sulfate, lithium acetate, lithium formate, bis-tris propane, glycerol, dithiothreitol, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 25-30 (mg/ml) | |
| 2 | 1 | drop | sodium phosphate | 10 (mM) | pH8.0 |
| 3 | 1 | reservoir | lithium sulfate | 1.5 (M) | |
| 4 | 1 | reservoir | lithium acetate | 0.15 (M) | |
| 5 | 1 | reservoir | lithium formate | 0.1 (M) | |
| 6 | 1 | reservoir | glycerol | 2 (%(v/v)) | |
| 7 | 1 | reservoir | dithiothreitol | 1 (mM) | |
| 8 | 1 | reservoir | Tris-propane | 0.1 (M) | pH8.0 |
